| AVS 61st International Symposium & Exhibition | |
| Biomaterial Interfaces | Monday Sessions |
| Session BI+AS-MoM |
| Session: | Biomolecules & Biomaterials Interfaces |
| Presenter: | Michael Zharnikov, University of Heidelberg, Germany |
| Authors: | M.N. Khan, University of Heidelberg, Germany V. Tjong, Duke University A. Chilkoti, Duke University M. Zharnikov, University of Heidelberg, Germany |
| Correspondent: | Click to Email |
We present here a versatile approach to prepare mixed monolayers of thiolate-bound single stranded DNA (ssDNA) and oligo(ethylene glycol) substituted alkanethiols (OEG-AT) in a broad range of compositions as well as ssDNA/OEG-AT patterns of desired shape embedded into a biorepulsive background. The procedure involves two steps. First, a OEG-AT monolayer on a solid support is exposed to electrons or UV light in either homogeneous or lithographic fashion. Second, the promoted (by the irradiation in the first step) exchange reaction between the damaged OEG-AT species in the film and ssDNA substituents in solution occurs, resulting in formation of a ssDNA/OEG-AT monolayer or pattern. The composition of the mixed films or ssDNA/OEG-AT spots (lithography) can be precisely adjusted by electron or UV dose in almost entire composition range. The above procedure relies on commercially available compounds and is applicable to both thiol-terminated and symmetric and asymmetric disulfide-terminated ssDNA. The fabricated OEG-AT/ssDNA templates and patterns can be extended into the z-dimension by surface-initiated enzymatic polymerization of ssDNA, which results in the formation of highly ordered ssDNA brushes and allows topographically complex ssDNA brush patterns to be sculpted on the surface.