AVS 60th International Symposium and Exhibition
    Scanning Probe Microscopy Focus Topic Thursday Sessions
       Session SP+AS+BI+MI+NS+SS-ThM

Invited Paper SP+AS+BI+MI+NS+SS-ThM11
Minimally Invasive AFM for Imaging Biomolecules in Liquid

Thursday, October 31, 2013, 11:20 am, Room 202 C

Session: Advances in Scanning Probe Imaging
Presenter: B.W. Hoogenboom, University College London, UK
Correspondent: Click to Email
Atomic force microscopy (AFM) is a unique tool in combining nanometre spatial resolution and high temporal resolution with the ability to visualise biological molecules in their native environment, i.e., aqueous solution. Its ultimate resolution on such samples depends on the strength of the interaction between the sample and the AFM probe: Too weak an interaction means low contrast, too high an interaction usually results in molecules being distorted or dislodged. I will discuss our recent work on minimising the invasiveness of AFM in liquid, resulting among others in the first observation of the DNA double helix on a single molecule in aqueous solution [Nano Lett. 2012, 12(7), pp. 3846-3850].