AVS 60th International Symposium and Exhibition
    Exhibitor Technology Spotlight Tuesday Sessions
       Session EW-TuA

Paper EW-TuA6
Picolitre Dosing of Proteins onto Biomaterials Microarrays to Monitor Cell Response in a Combinatorial Assay

Tuesday, October 29, 2013, 3:40 pm, Room Hall A

Session: Exhibitor Technology Spotlight III
Presenter: C. Dufresne, Scienion US, Inc.
Authors: C. Dufresne, Scienion US, Inc.
M. Hammad, University of Nottingham, UK
M.R. Alexander, University of Nottingham, UK
Correspondent: Click to Email
A high throughput model of protein pre-adsorption on biomaterials for control of cell adhesion and proliferation is being investigated using Scienion's non-contact low volume dispensing technology. Valuable proteins combinations (31 in triplicate) were piezo-dispensed and pre-adsorbed onto microarray spots representing seven biomaterials. Cell density correlates with the surface chemistry of the protein pre-adsorbed polymer spots as acquired by ToF-SIMS. It is only possible to rapidly produce and assess this many protein adsorption combinations with a high throughput platform combined with high throughput surface characterization. Details of this technology will be presented.