| AVS 60th International Symposium and Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuP |
| Session: | Biomaterials Interfaces Poster Session |
| Presenter: | M. Alles, University of Heidelberg, Germany |
| Authors: | M. Alles, University of Heidelberg, Germany C. Christophis, University of Heidelberg, Germany M.E. Callow, University of Birmingham, UK M.H. Grunze, University of Heidelberg, Germany A. Rosenhahn, Ruhr-University Bochum, Germany |
| Correspondent: | Click to Email |
In recent years Fouling-Release (FR) technologies have been significantly improved and can be considered as an environmental benign approach against marine biofouling [1]. FR coatings refer to those coatings on which microorganisms adhere only weakly allowing their release by low shear stresses present e.g. at the hull of a cruising ship. To study cell adhesion strength on different substrates quantitatively, a microfluidic shear force assay was developed [2-3]. After an attachment phase, the adhesion strength of cells can be measured by detaching them from substrates using a stepwise increased flow across 6 orders of magnitude starting with very low shear forces of 0.01 dyn·cm-2. With this device we can determine both, the fraction of adherent cells and the critical shear stress which is necessary to remove 50% of the adherent cells. Diatoms are frequently observed biofoulers and prevalent on fouling-release coatings commercially used [4]. In the presented work we tested the effect of different incubation conditions and attachment geometries on the attachment strength of the marine diatom Navicula perminuta. Furthermore we used chemically different substrates to determine if adhesion strength of Navicula is changed on potential foul-release chemistries. By this microfluidic approach, inert chemistries can readily be discriminated from surfaces with low foul release properties and the high sensitivity allows revealing even subtle differences in adhesion caused by a change in surface properties.
1. Callow, J.A. and M.E. Callow, Trends in the development of environmentally friendly fouling-resistant marine coatings. Nat Commun, 2011. 2: p. 244.
2. Christophis, C., M. Grunze, and A. Rosenhahn, Quantification of the adhesion strength of fibroblast cells on ethylene glycol terminated self-assembled monolayers by a microfluidic shear force assay. Physical Chemistry Chemical Physics, 2010. 12(17): p. 4498-4504.
3. Christophis, C., et al., Shear Stress Regulates Adhesion and Rolling of CD44+ Leukemic and Hematopoietic Progenitor Cells on Hyaluronan. Biophysical Journal, 2011. 101(3): p. 585-593.
4. Zargiel, K.A., J.S. Coogan, and G.W. Swain, Diatom community structure on commercially available ship hull coatings. Biofouling, 2011. 27(9): p. 955-965.