| AVS 60th International Symposium and Exhibition | |
| Biomaterial Interfaces | Thursday Sessions |
| Session BI+AS+BA+NS+SS-ThA |
| Session: | Biomolecules at Interfaces |
| Presenter: | A. Galtayries, Chimie ParisTech, France |
| Authors: | A. Galtayries, Chimie ParisTech, France A. Dellinger, Chimie ParisTech, France V. Semetey, Institut Curie, France |
| Correspondent: | Click to Email |
The control of biomolecules adsorption (such as proteins) and other microorganisms is of high interest for various fields of biotechnology, such as bioanalytics, cell biology, tissue engineering and biomaterials. A simple and efficient method to control adsorption includes the use of the thiolene chemistry to form self-assembled monolayer (SAM) from commercial long (poly(ethylene glycol)) and short (oligo-ethylene glycol) terminated chains, applied on metal oxide surfaces [1].
Both on silicon, titanium and iron-chromium substrates, we selected two polymers either with short or long chains: one is adhesive, the other one is non-adhesive once in interaction with solutions of biomolecules. As regards short-chain molecules, the adhesive O-(2-Mercaptoethyl)-O′-methyl-hexa(ethylene glycol) and the adhesive O-(2-Carboxyethyl)-O′-(2-mercaptoethyl) heptaethylene glycol further activated by reaction with N-hydroxysuccinimide (NHS) were selected for grafting strategies implying full surface grafting or adhesive/non adhesive patternings (100 micrometer-large bands or half-moon surfaces). Similarly, as long-chain molecules, poly(ethylene glycol) methyl ether with an average molecular weight of 5,000 have been used, adhesive ones being NH2-terminated.With such molecular selection, we performed a systematic study using surface characterization techniques such as X-ray Photoelectron Spectroscopy (XPS), Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) and Infra-Red Surface Spectroscopy (ATR-IRFT or PM-IRRAS): at different steps of the grafting process, as well as after interaction with protein solutions, surface qualitative as well as quantitative information where obtained to discuss the efficiency of these molecular strategies to build biointerfaces on metal oxide surfaces.
[1] ``A Facile and Versatile Approach to Design Self-Assembled Monolayers on Glass using Thiol-ene Chemistry'', B. Oberleitner, A. Dellinger, M. Déforet, A. Galtayries, A.-S. Castanet,V. Semetey, Chemical Communication, 49, 1615-1617 (2013).