| AVS 59th Annual International Symposium and Exhibition | |
| Thin Film | Thursday Sessions |
| Session TF+EM+SS-ThA |
| Session: | Applications of Self-Assembled Monolayers and Layer-by-Layer Assemblies |
| Presenter: | J.J. Figueroa, University of South Florida |
| Authors: | J.J. Figueroa, University of South Florida S. Magana, University of South Florida D. Lim, University of South Florida R. Schlaf, University of South Florida |
| Correspondent: | Click to Email |
Physical adsorption (solid –liquid interface) is known as a simple and rapid option to immobilize biomolecules on various surfaces. Proteins, receptors and antibodies are attached via physisorption to different surfaces by various attachment protocols. However, physical adsorption has been often labeled in the past with disadvantages like variability, reversibility and low surface density of immobilized biomolecules. In contrast, the presented research demonstrates that spray deposition with a pneumatic nebulizer can be used to immobilize fully functional and stable physisorbed antibody coatings on glass surfaces with high reproducibility.
The experiments were performed using a low flow concentric nebulizer (commonly used on mass spectrometry), regular glass slides as a substrate and E. coli O157:H7 antibody as prototypical test system. The antibody films were examined for functionality, specificity and shelf life. A series of films with varying thickness and deposition conditions was characterized with respect to functionality, mechanical stability, surface morphology and antibody density. The results demonstrate that the films are comparable to films prepared with the standard covalent attachment protocol (avidin-biotin). They show low denaturation or conformational changes, minimal loss during the rinsing process suggesting good attachment to the surface, and they perform as well with regard to sensitivity, specificity and shelf-life. The morphology studies suggest that the non-oriented attachment of the spray deposited antibodies (compared to the oriented attachment achieved with the covalent attachment scheme) is compensated by a higher antibody density enabled by the non-equilibrium spray deposition process.