AVS 59th Annual International Symposium and Exhibition
    Nanometer-scale Science and Technology Thursday Sessions
       Session NS-ThP

Paper NS-ThP7
Application of Rolling Circle Amplification and Thermal Dynamic Principle for Manipulating the Interparticle Spacing of Gold Nanopaticle Chains

Thursday, November 1, 2012, 6:00 pm, Room Central Hall

Session: Nanometer-scale Science and Technology Poster Session
Presenter: Y.-C. Ou, National Applied Research Laboratories, Taiwan, Republic of China
Authors: Y.P. Lu, National Applied Research Laboratories, Taiwan, Republic of China
M.Y. Lin, National Applied Research Laboratories, Taiwan, Republic of China
Y.-C. Ou, National Applied Research Laboratories, Taiwan, Republic of China
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Biomolecular nanotechnology draws a lot of attention in organizing DNA sequence, which can architecturally build up a nano-sized scaffold, because of their potential utility in nano-machines and biosensing. According to the DNA complementary base-pairing interactions and aptamer design, DNA backbone has further been used for the construction of a variety of geometric objects and anchoring sites for functionalized proteins or metals. A simple and applicable procedure to miniature nanostructures is expected to enhance the detection sensitivity of devices. It is important to construct the metallic nanoparticle chain with different interparticle spacing because the organized metallic nanoparticles self-assembled from DNA nanostructure may affect the photoelectric properties. We successfully generate well-controlled formation of long, flexible, one dimensional gold nanoparticle (AuNP) chains by employed DNA template for self-assembly AuNP based on rolling circle amplification (RCA) technique. For the purpose of controlling the interparticle spacing of AuNP, we report the construction of long chain DNA-AuNP with or without secondary structure of DNA scaffold by using of base-pairing strategy and thermal dynamic reaction. The heating process under appropriate temperature broke the intramolecular Watson-Crick interactions to form the hairpin structure. This ultimately allowed no AuNP modified primers to hybridize with the open region of single strand DNA backbone, hence increased the interparticle spacing between each AuNP. The effect of AuNP distance in optical and electronic properties can be designed by controlling the DNA sequence and thermal dynamic reaction, and thus changed the optical and electronically characters of AuNP-based nanostructures for many applications in optoelectronics and biosensing devices fabrication.