Paper NS-MoM11
Fabrication of Fe Doped Nano-engineered Matrix for Cholesterol Biosensor

Monday, October 29, 2012, 11:40 am, Room 12

In recent years, sol–gel chemistry has been a subject of intense investigations in the fields of chemical sensors and biosensors as it offers a cost effective route the fabrication of high surface area plate forms for sensitive biosensors. The inherent low temperature process of the technology, provide an attractive way for the immobilization of heat-sensitive biological entities (enzyme, protein, and antibody). This class of sol–gel silica (TiO₂) matrix possesses chemical inertness, non-toxic, physical rigidity, tunable porosity, high photochemical and thermal stability, and optical transparency.

Nanostructured nature of the metal-oxides films have been extensively reported, we have utilized this property of the material for effective enzyme adsorption per unit area to develop high sensitivity biosensors. We have further improved the matrix property to alter the surface properties (microstructure and charge transport) by Fe doping in the TiO ₂ film. The modified matrix has been characterized by XRD, XPS, FTIR, SEM. The Fe³⁺ ion doped TiO₂ matrix successfully introduces redox property in the electrode and provides enhanced electron communication features. Sensing response obtained by the bioelectrocatalytic oxidation of cholesterol by cholesterol oxidase. Chox/ Fe³⁺ ion doped TiO₂ /ITO electrode was studied using cyclic voltammetry (CV). These studies reveal that the Chox/ Fe³⁺ ion doped TiO₂ /ITO bio-electrode exhibits improved biosensing response as compared to Chox/ Undoped TiO₂ /ITO electrode. The results confirm promising application of the Fe³⁺ ion doped TiO₂ thin film matrix for the realization of efficient cholesterol biosensor.