| AVS 59th Annual International Symposium and Exhibition | |
| Biofilms and Biofouling: Marine Medical Energy Focus Topic | Thursday Sessions |
| Session MB+BI-ThM |
| Session: | Biofilms and Biofouling in Medicine |
| Presenter: | A. Galtayries, Chimie ParisTech (ENSCP), France |
| Authors: | A. Galtayries, Chimie ParisTech (ENSCP), France A. Dellinger, Chimie ParisTech (ENSCP), France V. Semetey, Institut Curie, France |
| Correspondent: | Click to Email |
The control of biomolecules adsorption (such as proteins) and other microorganisms is of high interest for various fields of biotechnology, such as bioanalytics, cell biology, tissue engineering and biomaterials. An efficient method to control adsorption includes the use of well-defined oligo(ethylene glycol)-terminated self-assembled monolayer (SAM). However, multiple processing steps are often required to prepare SAM onto substrate. To address these problems of surface modification, we have developed a simple method using the powerful thiolene reaction to prepare self-assembled monolayer on different metal oxides to control bioadhesion on surfaces starting from commercially available building blocks, and taking the advantage of the photoreaction to easily create adhesive and anti-adhesive patterns.
Such well-controlled grafting strategy has been applied to different metal oxides: from silicon substrates for the methodology set-up to model metal oxides formed on biocompatible metals and alloys. The obtained films are robust; the process is low-cost, simple, and efficient.
Surface characterization as X-ray Photoelectron Spectroscopy (XPS), Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) and Infra-Red Surface Spectroscopy (ATR-IRFT or PM-IRRAS) were used to check the surface composition at different steps of the reactions: the thiolene reaction, the PEG grafting, as well as after interaction with protein solutions: albumin for preliminary tests, and fibronectin, an adhesive protein present in the extra-cellular matrix. In addition to quantitative information, obtained by XPS, about the oxide composition and thickness at the different steps, and qualitative information, obtained by XPS and ToF-SIMS, surface patterning could be emphasized with ToF-SIMS chemical surface imaging.