AVS 59th Annual International Symposium and Exhibition
    Biointerphases Focus Topic: Bioimaging Wednesday Sessions
       Session BN+AS-WeA

Paper BN+AS-WeA12
Characterization of Nanoparticles Implanted into Tissues for Enhancement of Ion-Mobility Mass Spectrometry Surface Imaging of Sagittal Brain Sections

Wednesday, October 31, 2012, 5:40 pm, Room 23

Session: Bioimaging
Presenter: J.A. Schultz, Ionwerks, Inc.
Authors: E.K. Lewis, Ionwerks, Inc.
J.F. Moore, MassThink
T.F. Egan, Ionwerks, Inc.
B. Chen, Rice University
B. Brinson, Rice University
V.M. Womack, Ionwerks, Inc.
D. Barbacci, Ionwerks, Inc.
R. Hauge, Rice University
A.S. Woods, National Institute on Drug Abuse / IRP
J.A. Schultz, Ionwerks, Inc.
Correspondent: Click to Email
Our research previously included the first demonstration of implanting nanoparticles nanometers below the surface of a biological tissue to provide a completely new method MALDI imaging. Now, we are working towards combining optical histology and molecular surface imaging. We are currently using x-ray photoelectron spectroscopy (XPS) to characterize the nanoparticle coverage, composition, and the depth nanoparticles are implanted into our biological tissue(s). Nanoparticles were implanted at energies from 2-6kV, initial XPS depth profiles demonstrated they are implanted into tissue at depths from 10-30nm. The use of nanoparticulate implantation provides a basis from which future surface imaging techniques can be developed and tested. This method is important in that not only are we preserving the optical histology, and location of lipids and peptides, but MALDI signals are increased on the order of 2-4 times over typical preparation with several orders of magnitude less matrix.