| AVS 59th Annual International Symposium and Exhibition | |
| Biointerphases Focus Topic: Bioimaging | Wednesday Sessions |
| Session BN+AS-WeA |
| Session: | Bioimaging |
| Presenter: | A. Rosenhahn, Ruhr-University Bochum, Germany |
| Authors: | A. Rosenhahn, Ruhr-University Bochum, Germany T. Gorniak, Karlsruhe Institute of Technology, Germany T. Senkbeil, Karlsruhe Institute of Technology, Germany A. Buck, Karlsruhe Institute of Technology, Germany M. Beckers, Karlsruhe Institute of Technology, Germany M.H. Grunze, Karlsruhe Institute of Technology, Germany |
| Correspondent: | Click to Email |
Coherent X-ray microscopy of hydrated biological samples – especially in the 'water window' at photon energies of 284–540 eV – is of tremendous interest for life sciences due to the high contrast of organic matter with respect to the aqueous background. We present recent progress in imaging of biological samples with coherent X-ray microscopy (holography and ptychography) and scanning X-ray nano/microprobe imaging. The main goal is application of these emerging techniques to the in-situ analysis of vitrified biological specimen. Ptychography uses coherent diffraction patterns at different sample positions while maintaining a fixed spatial overlap between the fields of view. By introducing this spatial redundancy to the data an additional constraint for the iterative reconstruction algorithm is achieved. This enhances the convergence of phase retrieval drastically and allows imaging of extended samples. We supplement these results with resonant ptychographic imaging at the oxygen K-edge core level resonances of test particles where both, absorption and phase shifts, revealed information about the chemical composition of the samples. First results on imaging of frozen hydrated specimen and samples in the liquid environment will be shown and discussed.