AVS 59th Annual International Symposium and Exhibition
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP5
Nanoscale Characterization of Acid and Thermally Treated Collagen Fibrils and its Effects on the Cellular Responses of Osteoblast

Tuesday, October 30, 2012, 6:00 pm, Room Central Hall

Session: Biomaterial Interfaces Poster Session
Presenter: Y.J. Park, KAIST, Republic of Korea
Authors: Y.J. Park, KAIST, Republic of Korea
G.J. Choi, KRISS, Republic of Korea
S.H. Kim, KRISS, Republic of Korea
J.H. Hahn, KRISS, Republic of Korea
T.G. Lee, KRISS, Republic of Korea
W.J. Lee, KAIST, Republic of Korea
D.W. Moon, KRISS, Republic of Korea
Correspondent: Click to Email
Type I collagen is a major extracellular matrix component and its hierarchical structure plays an important role in the regulation of cellular behavior. In order to study the effect of structure, surface chemistry, and mechanical properties change of collagen fibril on the cellular response, various collagen structures were prepared by different degrees of acidic and thermal treatment of native collagen fibrils. First, to study the microstructure and morphology of collagen, atomic force microscopy (AFM) was used due to its high spatial resolution and surface morphology specificity. Second, we applied time-of-flight secondary ion mass spectrometry (ToF-SIMS) to study the surface chemistry changes of collagen fibrils by utilizing the capability of providing molecular surface chemical information. Third, to observe the changes in the mechanical properties during acidic and thermal treatment of collagen fibrils, contact-resonance force microscopy (CR-FM) was applied because of its ability to provide not only nanoscale spatial resolution but also quantitative information about the mechanical properties. It was demonstrated that the change of microstructure, surface chemistry, and mechanical property of collagen induced by acidic and thermal treatment could be observed in molecular level using AFM, ToF-SIMS, and CR-FM. The structural, chemical, and mechanical properties of acid and thermally treated collagen fibrils could be correlated with the cellular responses such as cell morphology, cytoskeleton organization, and viability.