AVS 59th Annual International Symposium and Exhibition
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP4
Surface Topographic Patterns Functionalized with Different Biomaterials for Studying Neural Cell Behaviors

Tuesday, October 30, 2012, 6:00 pm, Room Central Hall

Session: Biomaterial Interfaces Poster Session
Presenter: Y.P. Lu, National Applied Research Laboratories, Taiwan, Republic of China
Authors: Y.P. Lu, National Applied Research Laboratories, Taiwan, Republic of China
M.Y. Lin, National Applied Research Laboratories, Taiwan, Republic of China
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Micro- and Nano-patterned substrates functionalized with extracellular matrix (ECM) have been recognized as powerful tools for regulating cell behaviors and functions, because biomimetic features enable the study of cellular responses to specific external stimulations. Surface topography in micro- or nano-scale contributes to provide a physical niche to resemble the physiological environment, whereas biomolecules in ECM can provide a cell-favorable environment in the artificial materials. Independent combination of topographical fabricated scaffolds and biomaterials with bioactive features have provided a suitable in vitro cellular function study system. We developed two different characteristics of polymer chips with microstructure and functionalized peptide, including polydimethylsiloxane (PDMS) chip modified with poly-D-lysine (PDL) peptide on the surface and silicon wafer modified with laminin-1 peptide on the surface. The PDMS chip was composed of ridges and grooves around 10 μm in width to form a stripe pattern with micro-meter scale. Another silicon substrate was prepared from micro-meter stripe pattern with nanorods in the grooves region. Neuron-like PC12 cells were then cultured on these 3D substrates and stimulated to manifest different behaviors, and induced cell differentiation with nerve growth factor (NGF) treatment. PC12 cells were cultured in biomimetic substrates impacted in several properties: Cells displayed contact guidance on both substrates and became elongated along the grating axis of scaffolds. When neuron cells cultured on the PDMS substrate, soma and neurite grew on the ridge, groove, or even lateral wall and formed the overlappin g distribution. On the other hand, PC12 cells grew on the nanorod substrate functionalized with laminin and displayed contact guidance and became parallel elongated along the flat ridge plane. Some neurites were able to cross groove through the nanorod-supported laminin bridge. Results gained from this study provide the manipulation of neuron cell fate by using enhanced patterning techniques and would be valuable in various biomedical applications, including tissue engineering, neuron regeneration, and basic cell biology.