Paper BI-TuP14
A Microfluidic Study of the Interaction of Haematopoietic Stem Cells with their Microenvironment
Tuesday, October 30, 2012, 6:00 pm, Room Central Hall
| Session: |
Biomaterial Interfaces Poster Session |
| Presenter: |
M. Hanke, Institute for Functional Interphases, KIT, Karlsruhe, Germany |
| Authors: |
M. Hanke, Institute for Functional Interphases, KIT, Karlsruhe, Germany
C. Christophis, Institute for Functional Interphases, KIT, Karlsruhe, Germany
C. Leinweber, Institute for Functional Interphases, KIT, Karlsruhe, Germany
N. Baran, University Hospital Heidelberg, Inner Medicine V, Germany
I. Taubert, University Hospital Heidelberg, Inner Medicine V, Germany
P. Wuchter, University Hospital Heidelberg, Inner Medicine V, Germany
A. Ho, University Hospital Heidelberg, Inner Medicine V, Germany
A. Rosenhahn, Institute for Functional Interphases, KIT, Karlsruhe, Germany
|
| Correspondent: |
Click to Email |
A microfluidic adhesion assay has been developed to quantitatively investigate the interaction of cells with interfaces under well defined flow conditions.[1] The device was applied to the study of the interaction of leukaemic cells and haematopoietic stem cells with hyaluronic acid surfaces. We found that beyond a critical shear stress the cell surface receptor CD44 mediates a catch bond, flow induced rolling of the cells on the surfaces[2], similar as observed for leukocytes during the extravasation process.[3] A similar rolling phenomenon occurred on mesenchymal stroma cells, which are present in the bone marrow niche creating the microenvironment required for haematopoietic stem cells to endlessly proliferate. The mesenchymal stroma cells inter alia secrete the stroma-cell-derived factor-1 alpha which has been reported to activate stem cell migration, mobilization and homing.[4] The effect of this chemokine on the movement of haematopoietic stem cells was also studied utilising a novel microstructured niche model.
[1] C. Christophis, M. Grunze, A. Rosenhahn, PCCP 2010, 12, 4498.
[2] C. Christophis, I. Taubert, G. Meseck, M. Schubert, M. Grunze, A. D. Ho, A. Rosenhahn, Biophys. J. 2011, 101, 585.
[3] L. Q. Jin, K. J. Hope, Q. L. Zhai, F. Smadja-Joffe, J. E. Dick, Nature Med. 2006, 12, 1167
[4] Jing DH, Fonseca AV, Alakel N, et al. Haematol-Hematol J. 2010, 95, 542-550.