AVS 59th Annual International Symposium and Exhibition
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP12
In Vitro Protein-Biofilm on Nanoparticles Characterizated by ToF-SIMS, STEM and TEM

Tuesday, October 30, 2012, 6:00 pm, Room Central Hall

Session: Biomaterial Interfaces Poster Session
Presenter: H.P. Wiesmann, Technische Universität Dresden, Germany
Authors: H.P. Wiesmann, Technische Universität Dresden, Germany
J. Neunzehn, Technische Universität Dresden, Germany
F. Draude, University of Muenster, Germany
H.F. Arlinghaus, University of Muenster, Germany
Correspondent: Click to Email
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was applied to detect and characterize different nano scaled protein coatings on gold nanoparticles. After washing the nanoparticles by various steps, the gold particles (diameter of about 10 to 20 nm) were coated with the proteins collagen type I and fibronectin and also different protein combinations in thin mono layers. The different nano-scaled protein layers on the cleaned gold nanoparticle surfaces were identified by detection of the protein typical amino acid mass peaks by time-of-flight secondary ion mass spectrometry.

In addition, the protein-coated particles were investigated by transmission electron microscopy to get information about the proteins structure and their layer thickness on the particle surfaces. It was possible to distinguish the protein coatings by their molecule thicknesses and to evaluate the different particle agglomeration influenced by the used proteins by the use of scanning transmission electron microscopy.