AVS 59th Annual International Symposium and Exhibition
    Biomaterial Interfaces Monday Sessions
       Session BI-MoM

Paper BI-MoM6
Binary Colloidal Crystal Structures Combined with Chemical Surface Modification to Achieve Superior Control Over Biointerfacial Interactions

Monday, October 29, 2012, 10:00 am, Room 23

Session: Surfaces to Control Cell Response
Presenter: P. Koegler, Swinburne Univ. of Tech., Australia
Authors: P. Koegler, Swinburne Univ. of Tech., Australia
P. Pasic, CSIRO Materials Science and Eng., Australia
J. Gardiner, CSIRO Materials Science and Eng., Australia
V. Glattauer, CSIRO Materials Science and Eng., Australia
A. Clayton, Swinburne Univ. of Tech., Australia
H. Thissen, CSIRO Materials Science and Eng., Australia
P. Kingshott, Swinburne Univ. of Tech., Australia
Correspondent: Click to Email
Biointerfacial interactions play a major role in the field of biomedical materials and regenerative medicine and are of tremendous importance to in vivo and in vitro applications. Cell-material interactions are mediated by surface parameters including the materials surface chemistry and topography. Colloidal lithography represents a promising tool to modify surface topographies at the nanoscale with precision and over large areas while at the same time not requiring complex instrumental set-ups or rigorous experimental conditions. The creation of nanostructured surfaces in this way can also be combined with sophisticated surface modification techniques such as polymer grafting techniques via functional groups (grafting-to) or initiating groups (grafting-from) on the particle surface. This platform, which provides control over surface chemistry and topography, offers great flexibility in regard to the design of advanced surface coatings.In the current study we have generated highly ordered binary colloidal crystal structures using surface functionalized particles. This approach allows precise control over particle size, spacing, and thus pattern morphology. In order to minimize undesired non-specific protein adsorption which can mediate cell attachment, graft polymer coatings were applied to particles using heterobifunctional poly(ethylene glycol) (PEG) to render the surfaces non-fouling. In addition, colloid crystal modified surfaces were modified with specific bioactive signals, such as the cyclic RGD peptide (cRGDfK) to promote cell attachment. Surface characterization was carried using scanning electron microscopy (SEM), atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). Cell culture experiments were carried out using L929 mouse fibroblasts up to 24 hours.The unprecedented control over the surface chemistry and topography provided by this simple coating platform is of significant interest for the study of biointerfacial interactions and the development of new and improved biomedical devices.