| AVS 59th Annual International Symposium and Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI+SS+AS-TuM |
| Session: | Biomolecules at Interfaces |
| Presenter: | T. Arnebrant, Malmö University, Sweden |
| Authors: | T. Arnebrant, Malmö University, Sweden L. Lindh, Malmö University, Sweden J. Sotres, Malmö University, Sweden |
| Correspondent: | Click to Email |
Adsorbed salivary protein layers will cover soft and hard surfaces in the oral cavity, where they fulfill a protective function influencing adhesion and wear, and also surfaces of devices exposed to saliva. Properties of salivary films will depend on the characteristics of the surface on which they are formed as well as solution conditions (salt, pH) and will affect surface properties such as wettability and charge. Moreover, normal and lateral forces between surfaces bearing salivary films will be distinctly different than for bare surfaces. Such changes in surface properties and interactions may be relevant not only for events at oral interfaces but also for the operation of monitoring or sampling devices immersed in or exposed to saliva. Here, we show how a combined characterisation of these systems through different surface techniques provides important information on the role of this body fluid which is not available through more common chemical or biochemical approaches. The presentation will describe adsorption characteristics of salivary proteins from the total secretion as well as for purified fractions including single protein preparations. Influence by surface properties and ambient (solution) conditions will be outlined. Data on structure of salivary films as obtained by in situ ellipsometry, QCM-D and neutron reflectivity will be reported. Furthermore, SFA and AFM measurements of DLVO, steric, adhesive and frictional forces between surfaces bearing salivary films will be discussed. A new method for estimating the strength of salivary films based on simultaneous recording of roughness and friction data from AFM will also be described.
References: Protein Adsorption in the Oral Environment, Arnebrant T, In Biopolymers at Interfaces 2nd ed. (M. Malmsten Ed.) Marcel Dekker, 2003, pp 811-856Friction force spectroscopy as a Tool to Study the Strength and Structure of Salivary Films. Sotres J., Liselott L., Arnebrant T. 2011. Langmuir, 27 (2011), 13692-13700.