| AVS 59th Annual International Symposium and Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI+AS-TuA |
| Session: | Characterization of Biointerfaces |
| Presenter: | L. Hanley, University of Illinois at Chicago |
| Authors: | M. Blaze, University of Illinois at Chicago C. Bhardwaj, University of Illinois at Chicago A. Akhmetov, University of Illinois at Chicago L. Hanley, University of Illinois at Chicago |
| Correspondent: | Click to Email |
Bacterial biofilms are structured communities of microbes encapsulated within a self-developed polymeric matrix which adhere to surfaces and display genetic expression distinct from freely floating bacteria. Biofilms are frequently found to populate medical devices, leading to significant problems of infection in the first few days after implantation. Polyelectrolyte multilayers are developed for the delayed delivery of antibiotics to inhibit biofilm growth on biomedical devices [1]. Ten layers each of chitosan and alginate are prepared on a gold substrate, then infused with a novel antibiotic compound. This antibiotic-infused multilayer is found to inhibit the growth of Enterococcus faecalis bacterial biofilms on membranes over an 18 hour exposure. Laser desorption postionization mass spectrometry (LDPI-MS) is used to characterize the antibiotic after synthesis [2]. LDPI-MS analysis shows that the antibiotic survives sterilization of the multilayer surface, but <1% of the antibiotic remains after exposure to the biofilm.
[1] M. Blaze M.T., L.K. Takahashi, J. Zhou, M. Ahmed, G.L. Gasper, F.D. Pleticha, and L. Hanley, Anal. Chem. 83(2011) 4962.
[2] A. Akhmetov, J.F. Moore, G.L. Gasper, P.J. Koin, L. Hanley, J. Mass Spectrom. 45 (2010) 137.