Invited Paper BI+AS-TuA11
Microfluidic Devices for High-Throughput Quantitation in Biology: From Biophysics to Diagnostics

Tuesday, October 30, 2012, 5:20 pm, Room 23

Microfluidic devices promise to have a significant impact on human health, particularly in diagnostics and drug development. We have developed a suite of microfluidic devices for high-throughput protein biochemistry and applied them to a wide range of applications spanning from protein biophysics to diagnostics, drug development, and vaccine development. Here I will discuss a novel approach to obtaining hundreds of kinetic rate measurements of bimolecular interactions on a single microfluidic device. In a second example I will present a generic microfluidic platform capable of quantitating biomarkers from a wide variety of samples in high-throughput and ultra-low cost, which could ultimately supersede the classical ELISA assay. We applied this platform to vaccine development by quantitating the activation of dendritic cells in response to a large panel of binary adjuvant combinations.