AVS 58th Annual International Symposium and Exhibition
    Biofabrication and Novel Devices Focus Topic Tuesday Sessions
       Session BN-TuP

Paper BN-TuP3
Towards F1-ATPsynthase Based Hybrid Nanobiodevice Fabrication

Tuesday, November 1, 2011, 6:00 pm, Room East Exhibit Hall

Session: Biofabrication and Novel Devices Poster Session
Presenter: Jenifer K. Settle, University of Kansas
Authors: J.K. Settle, University of Kansas
M.L. Richter, University of Kansas
C.L. Berrie, University of Kansas
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Incorporation of biomolecules into nanoscale devices, termed nanobiodevices, requires control over biomolecule placement within the device. Nonspecific adsorption of the tiny molecular motor, F1-ATPsynthase (ATPase), results in a variety of protein orientations on the surface. To improve the functionality of the immobilized protein, only one orientation (gamma unit upright) is desired. Therefore, controlling orientation is also imperative in increasing the number of functional molecules. Several techniques have been utilized to study and control this adsorption process. Atomic force microscopy was used to graft a dithiol into a resist matrix monolayer, exposing a thiol group. Through maleimide chemistry, a maleimide-nitriloacetic acid (NTA) group can be attached to the terminal end of the dithiol pattern. NTA will coordinate with nickel ions, which then coordinates with the histidine tag on the ATPase, thus controlling orientation. This process has also been studied via surface plasmon resonance. By coordinating these efforts with nanoelectrode construction, a functional nanobiodevice may be engineered.