| AVS 58th Annual International Symposium and Exhibition | |
| Biomaterial Interfaces Division | Monday Sessions |
| Session BI-MoM |
| Session: | Biomolecules at Interfaces |
| Presenter: | Ishtiaq Saaem, Duke University |
| Authors: | I. Saaem, Duke University A. Marchi, Duke University J. Tian, Duke University T. LaBean, Duke University |
| Correspondent: | Click to Email |
High-throughput synthesis of quality mixed oligonucleotides (oligos) allows for exhaustive studies of DNA nanostructured material formation. In situ DNA synthesis was achieved with a custom piezoelectric inkjet system using phosphoramidite chemistry, on functionalized cyclic olefin copolymer (COC) as a chip substrate. When amplified off of the chip via strand displacement amplification (SDA), the oligos were eluted and used directly for constructing multiple DNA origami nanostructures in a one-pot assembly. By performing SDA, each chip may be reused multiple times to produce pools of staple strands for repeated experiments. Two separate DNA origami structures were formed from a double-stranded scaffold strand. By redesigning specific staple strands, these structures can be connected to provide a larger nanostructured template.