| AVS 57th International Symposium & Exhibition | |
| Nanometer-scale Science and Technology | Thursday Sessions |
| Session NS+BI-ThA |
| Session: | Biomolecular Templates & Bioinspired Nanomaterials |
| Presenter: | M. Palma, Columbia University |
| Authors: | M. Palma, Columbia University J.J. Abramson, Columbia University E. Penzo, Columbia University A. Gorodetsky, Columbia University R. Wang, Columbia University M.P. Sheetz, Columbia University C. Nuckolls, Columbia University J. Hone, Columbia University S.J. Wind, Columbia University |
| Correspondent: | Click to Email |
The ability to control biomolecules on surfaces with nanometer resolution is of great interest in the field on nanoscience and nanotechnology. DNA nanoarrays, in particular, are of interest in the study of DNA-protein interactions, for biodiagnostic investigations and as a tool to drive self-organization of nanomoieties on surfaces. In this context, achieving a highly specific nanoscale assembly of oligonulceotides at surfaces is critical.
Here we describe different strategies to control the immobilization of single- and double-stranded DNA, as well as DNA nanostructures (DNA “origami”), on nanopatterned surfaces, with features down to the sub-10nm regime.
Using electron-beam and nanoimprint lithography we fabricated sub-10nm metal dots arranged in multiple configurations on Si or glass substrates. We have developed strategies for the selective bio-functionalization of these patterns, at the single nanodot level: each step of the biochemical functionalization has been monitored by Fluorescence Microscopy. The bio-functionalization approach used allowed for the formation of non-sterically hindered DNA nanodomains where the dsDNA attached to the dots maintains its native conformation, as confirmed by restriction enzymes studies. This allowed us, moreover, to follow the activity (at surfaces) of a restriction enzyme in real time and at the nanoscale: the monitoring of protein-DNA interactions with such biological nanoarrays will be discussed.
We will highlight the broader utility and application of such nanopatterned surfaces for the self-organization of DNA nanostructures. In-situ hybridization between the complementary strands on DNA nanostructures and on functionalized nanodots has been achieved, resulting in the ordered placement of the origami on the dot patterns, as demonstrated by Atomic Force Microscopy (AFM) imaging, both in liquid and in air.
Finally, we will discuss the application of DNA origami as functional scaffolds for the assembly of different nanomoieties (e.g Au nanoparticles and carbon annotubes): highly complex arrangements can be created with high resolution and high throughput, opening the possibility for the realization of electronic devices at the molecular scale.