| AVS 57th International Symposium & Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI2+AS-TuA |
| Session: | Combining Techniques for Biointerface Characterization |
| Presenter: | G. Mishra, Kratos Analytical Ltd., UK |
| Authors: | G. Mishra, Kratos Analytical Ltd., UK A.J. Roberts, Kratos Analytical Ltd., UK D.J. Surman, Kratos Analytical Ltd. S.L. McArthur, Swinburne University of Technology, Australia |
| Correspondent: | Click to Email |
Analysis of biomolecules on surfaces is essential to various applications of biosensors and biomolecule engineering. Matrix-assisted laser desorption/ionisation (MALDI) is now a well established technique for mass spectrometry of biomolecules. Different matrix-analyte preparation protocols have been shown to influence the desorption or ablation process resulting in either high or low metastable fragmentation. It has been speculated that following laser ablation the velocities of the analyte and matrix can be regarded as a valuable and meaningful characteristic of the MALDI process. However, the interaction and distribution of the analyte with respect to the matrix is poorly understood. Here we study the distribution of a selection of biomolecules as a function of matrix material using high resolution imaging x-ray photoelectron spectroscopy (XPS). We feel that understanding the depth distribution of biomolecules in the matrix remains factor which would enable us to draw conclusions on the incorporation of the analyte in the matrix. Thus in this study we also investigate the depth distribution using organic depth profiling techniques.