| AVS 57th International Symposium & Exhibition | |
| Biomaterial Interfaces | Tuesday Sessions |
| Session BI-TuM |
| Session: | Cells on Surfaces |
| Presenter: | F. Rossi, Joint Research Centre, Italy |
| Authors: | A. Ruiz, University Milan, Italy L.M. Buzanska, Polish Academy of Sciences, Poland M. Zychowicz, Polish Academy of Sciences, Poland P. Colpo, Joint Research Centre, Italy F. Rossi, Joint Research Centre, Italy |
| Correspondent: | Click to Email |
This work relates to a method of patterning human neural stem cells as a suitable platform for performing different studies of cell proliferation, migration, and differentiation. The patterning of cells has been achieved by using microcontact printing (MCP) to create micropatterns of Poly-L-Lysine (PLL) and Fibronectin (FN), on substrates coated with cell repellent poly ethylene glycol (PEG) deposited by plasma. This substrate is particularly interesting since it is anti adhesive in liquid, but protein adhesive in the dry state. More details of the preparation method are described in [1]. Briefly, microstructured polydimethilsiloxane stamps have been fabricated by casting silicon masters produced by photolithography. The PDMS stamps have been inked with a FN or PLL solution, then dried with a nitrogen stream and put in conformal contact with the PEG substrate. By this method we are able to create fouling (PLL, FN) / antifouling (PEG) contrast on the surfaces where the cells are incubated and by modification of the spot size and distance, the influence of the cell environment on stem cells maintenance and fate studied.
Human Umbilical Cord Blood - Neural Stem Cells [2] were grown on the platforms with PLL or fibronectin pattern. After incubation for 4 days on PLL patterns consisting of 105µm squares spaced 300 µm, the cells are predominantly localized within the square. Such behavior is conditioned by agents added to the incubating medium. After being exposed to dBcAMP, the cells extend neuronal projections outside the squares, but cell bodies are patterned within the active domain. Immunocytochemistry was applied to trace neuronal lineage specific markers and their redistribution on the pattern domain upon influence of differentiating agents. It is found that the maintenance and fate of stem cells can be controlled by a combination of the protein type layer deposited by MCP and culture medium composition: presence of serum, neuromorphogenes and growth factors. Proper arrangement of soluble factors and bioactive surface domains allowed to work out conditions for developmental stage-specific immobilization of neural stem cells to the surface.
1. A. Ruiz, L. Buzanska, D. Gilliland, T. Sobanski, L. Ceriotti, S. Coecke, P. Colpo, F. Rossi. Micro-stamped surfaces for the patterned growth of neural stem cells. Biomaterials 29 (2008) 4766-4774.
2. Buzanska, L., Jurga, M., Stachowiak, E.K., Stachowiak, M.K., Domanska-Janik, K. Stem cells Dev., 15, 391–406, 2006.