| AVS 57th International Symposium & Exhibition | |
| Biomaterial Interfaces | Thursday Sessions |
| Session BI-ThP |
| Session: | Biomaterial Interfaces Poster Session |
| Presenter: | S.-Y. Hsiao, Instrument Technology Research Center, NARL, Taiwan |
| Authors: | S.-Y. Hsiao, Instrument Technology Research Center, NARL, Taiwan Y.-S. Lin, Hungkuang University, Taiwan Y.-P. Lu, Instrument Technology Research Center, NARL, Taiwan C.-Y. Lin, Taipei Medical University, Taiwan H.-S. Huang, Instrument Technology Research Center, NARL, Taiwan H.-M. Huang, Taipei Medical University, Taiwan D.-C. Chen, National Yang-Ming University, Taiwan T.-S. Liao, Instrument Technology Research Center, NARL, Taiwan J.-S. Kao, Instrument Technology Research Center, NARL, Taiwan |
| Correspondent: | Click to Email |
An electrochemical impedance spectroscopic (EIS) instrument with indium-tin oxide (ITO) culture chip module and lock-in impedance readout module was developed. The lock-in impedance readout module achieves impedance measurements with a small, bio-harmless AC signal. Moreover, transparent ITO culture-chip module is experience friendly; other optical inspections can be applied on the chip. The impedance readout module is designed to perform in a range from 1 to 10 kHz; the phase measuring errors are within 5.1% in that range. Typical examples of PBS solutions, BSA proteins and cell culturing tests are discussed in experiments. Higher concentration levels of PBS produced lower impedance. Higher concentration level of BSA solution also produced lower impedance. Furthermore, experiments of 0.25% BSA dissolved in sDDW and 1X PBS show that the mediums influence system impedance. Moreover, the log phase (period of cell proliferation) of B16F10 cell culture tests ended at 9, 16, 23, and 65 h for seeded cell densities of 5×104, 1×105, 2×105, and 4×105/mL, respectively. Finally, cultures are incubated and doped to demonstrate the monitoring of cell proliferation.
An EIS using microelectrode arrays has gained much attention as a promising, label free, fast, and real-time method for cellular analysis. The electric cell substrate impedance sensing (ECIS; Applied BioPhysics) and real time cell electronic sensing (RT-CES; Roche Applied Sciences) systems are widely applied in measuring cell proliferation, attachment and spreading, motility, toxicology, barrier function, wounding, and migration. The sensing chips are opaque and the influence of solutions is generally disregarded. However, transparent chips for optically related detection are required in many applications. And, in fact, chemical additives for biochemical treatments induce impedance variations.
This study develops an EIS instrument with an ITO culture chip module and lock-in impedance readout module. The instrument can apply and extract bio-harmless small signals by lock-in technology. Experimental results prove impedance readout module performance. The ITO culture chip module electrodes are connected via the electrolyte medium and the route of cell/tissue. Typical examples of the effects of solutions and cell culturing tests are discussed. A higher level of PBS causes lower impedance. Further study of solution ingredients will improve the measurement accuracy and results analysis. The ESI instrument with an ITO culture chip module and lock-in impedance readout module can be applied in other conductive liquid sample investigations.