| AVS 57th International Symposium & Exhibition | |
| Biomaterial Interfaces | Thursday Sessions |
| Session BI-ThP |
| Session: | Biomaterial Interfaces Poster Session |
| Presenter: | A. Gölzhäuser, University of Bielefeld, Germany |
| Authors: | L. Kankate, University of Bielefeld, Germany H. Großmann, Johann Wolfgang Goethe-University, Germany U. Werner, University of Bielefeld, Germany R. Tampé, Johann Wolfgang Goethe-University, Germany A. Turchanin, University of Bielefeld, Germany A. Gölzhäuser, University of Bielefeld, Germany |
| Correspondent: | Click to Email |
Protein resistant oligo(ethylene glycol) terminated (OEG) self-assembled monolayers (SAMs) of thiols on gold are commonly used for suppression of nonspecific protein adsorption in biology and biotechnology. The standard preparation for these SAMs is the solution method (SM) that involves immersion of the gold surface in an OEG solution. Here we present the preparation of 11-(mercaptoundecyl)-triethylene glycol (HS(CH2)11(OCH2CH2)3OH) SAMs on gold surface by vapor deposition (VD) in vacuum. We compare the properties of SAMs prepared by VD and SM using X-ray photoelectron spectroscopy (XPS), polarisation modulation infrared reflection absorption spectroscopy (PM-IRRAS) and surface plasmon resonance (SPR) measurements. VD and SM SAMs exhibit similar packing density and show a similar resistance to the nonspecific adsorption of various proteins (bovine serum albumin, trypsin, and myoglobin) under physiological conditions. A very high sensitivity of the OEG SAMs to X-ray radiation is found, which allows tuning their protein resistance. These results show a new path to in situ engineering, analysis and patterning of protein resistant OEG SAMs by high vacuum and ultra high vacuum techniques.
[1] L. Kankate, H. Großmann, U. Werner, R. Tampé, A. Turchanin, A. Gölzhäuser, Biointerphases 5, 30 (2010)