| AVS 56th International Symposium & Exhibition | |
| Nanometer-scale Science and Technology | Tuesday Sessions |
| Session NS-TuP |
| Session: | Nanometer-scale Science and Technology Poster Session |
| Presenter: | X. Wang, Portland State University |
| Authors: | X. Wang, Portland State University O. Ramström, KTH - Royal Institute of Technology, Sweden M. Yan, Portland State University |
| Correspondent: | Click to Email |
Biofunctionalized metal nanoparticles provide a sensitive platform for the analysis and detection of molecular recognitions in biological system. The remarkably sensitive optoelectronic properties of metal nanoparticles make nanoparticle-based materials a powerful tool to study fundamental biorecognition processes. Here we present a new and versatile method for coupling underivatized carbohydrates to gold nanoparticles (Au NPs) via the photochemically induced reaction of perfluorophenylazide (PFPA). A colorimetric model system based on the carbohydrate-modified Au NPs was furthermore constructed for the sensitive detection of carbohydrate-protein interactions. Treating mono-, and di-mannose-modified Au NPs with Concanavalin A (Con A) lead to a distinct color change of the Au NP solution, which was directly associated with the surface plasmon resonance shift upon nanoparticle aggregation. The coupling chemistry is highly efficient and is applicable to a wide range of carbohydrate structures.