| AVS 56th International Symposium & Exhibition | |
| Biomaterial Interfaces | Thursday Sessions |
| Session BI-ThP |
| Session: | Biomaterial Interfaces Poster Session II (Arrays, Sensing, Micro/Nanofabrication, SPM) |
| Presenter: | R. Dahint, University of Heidelberg, Germany |
| Authors: | R. Dahint, University of Heidelberg, Germany F.C. Liu, University of Heidelberg, Germany N. Waly, University of Heidelberg, Germany H.O. Guvenc, University of Heidelberg, Germany T. Felgenhauer, German Cancer Research Center, Germany F. Breitling, German Cancer Research Center, Germany M. Himmelhaus, University of Heidelberg, Germany |
| Correspondent: | Click to Email |
Recently, we developed a novel complex material with combined optical and biological functionality [1, 2]. It consists of dielectric nanoparticles, which are adsorbed onto a plain gold surface and subsequently metallized by deposition of gold colloid prior to electroless plating. Upon reflection of white light, the layers exhibit pronounced extinction peaks which shift to higher wavelengths when molecules adsorb onto the surface. For simple alkanethiols a significantly higher red-shift of the extinction maximum was observed than reported for conventional surface plasmon resonance. To detect biomolecular interactions in array format it is crucial to guarantee homogenous optical response of the nanoparticle layers on macroscopic scales. We will, therefore, discuss the impact of different coating procedures on the optical properties of the films. To optimize sensitivity, effects of particle layer density, dielectric interlayers and plating time have been investigated. We also compared the response of core-shell nanoparticle layers to the optical properties of surface adsorbed gold colloid films. The final goal is to incorporate high-density peptide arrays into the optically responsive nanoparticle films by combinatorial laser printer synthesis [3] to facilitate label-free high-throughput screening of biomolecular interactions for biomedical and pharmaceutical applications. For this purpose, the peptide probes are embedded into a protein resistant matrix based on poly(ethylene glycol)methacrylate (PEGMA). The stability of both nanoparticle layers and PEGMA coating has been optimized with respect to the chemical and physical requirements of the biomolecular coupling reactions.
[1] R. Dahint, E. Trileva, H. Acunman, U. Konrad, M. Zimmer, V. Stadler, M. Himmelhaus, Biosens. Bioelectron., 22, 3174-3181 (2007)
[2] P. Buecker, E. Trileva, M. Himmelhaus, R. Dahint, Langmuir, 24, 8229-8239 (2008)
[3] V. Stadler, T. Felgenhauer, M. Beyer, S. Fernandez, K. Leibe, S. Güttler, M. Gröning, K. König, G. Torralba, M. Hausmann, V. Lindenstruth, A. Nesterov, I. Block, R. Pipkorn, A. Poustka, F. R. Bischoff, F. Breitling, Angew. Chem. Int. Ed ., 47, 7132-7135 (2008)