AVS 56th International Symposium & Exhibition
    Biomaterial Interfaces Thursday Sessions
       Session BI-ThP

Paper BI-ThP10
Absolute Quantification of Bio-molecules Immobilized on Self-Assembled Monolayers

Thursday, November 12, 2009, 6:00 pm, Room Hall 3

Session: Biomaterial Interfaces Poster Session II (Arrays, Sensing, Micro/Nanofabrication, SPM)
Presenter: H. Min, KRISS, Rep. of Korea
Authors: H. Min, KRISS, Rep. of Korea
Y.J. Lee, KRISS, Rep. of Korea
D.W. Moon, KRISS, Rep. of Korea
T.G. Lee, KRISS, Rep. of Korea
Correspondent: Click to Email
Biochips such as DNA and protein chips are becoming increasingly important in molecular diagnostics due to their low cost and the need for automated and easy-to-handle techniques. However, only a fraction of biochip products are approved by the FDA for clinical purposes because of the demand for accurate and reproducible biochip performance that can also be quantified. In this study, we develop a new method for the absolute quantification of the probe molecules (DNA, PNA and protein) immobilized on self-assembled monolayers (SAMs) by using medium energy ion scattering (MEIS) spectroscopy. In addition, measuring the amounts of target molecules in interaction with probe molecules on biochip surfaces, we determined the hybridization efficiencies of the DNA-DNA and PNA-DNA systems or the interaction efficiency of the protein-protein system. Our results show that this new methodology would be very useful for quality control of biochips in bio-medical applications.