AVS 56th International Symposium & Exhibition
    Biomaterial Interfaces Thursday Sessions
       Session BI-ThP

Paper BI-ThP1
Integration of Protein Microarrays and Glyconanoparticles with Surface Plasmon Resonance Imaging to Probe Carbohydrate-Protein Interactions

Thursday, November 12, 2009, 6:00 pm, Room Hall 3

Session: Biomaterial Interfaces Poster Session II (Arrays, Sensing, Micro/Nanofabrication, SPM)
Presenter: H. Wang, Portland State University
Authors: H. Wang, Portland State University
X. Wang, Portland State University
M. Yan, Portland State University
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Multivalent interactions between carbohydrates and proteins are attracting increasing interest because of their importance in many biological processes. We developed a strategy integrating microarrays, nanoparticles and surface plasmon resonance imaging (SPRi) to study carbohydrate-protein interactions. Protein microarrays were fabricated and carbohydrate-coated glyconanoparticles were used as multivalent probes to target carbohydrate-binding proteins. SPRi offers a real-time sensing method to investigate multiple binding events simultaneously. The chemistry, fabrication and characterization of microarray platforms will be discussed. The binding parameters and SPRi responses with regard to surface chemistry and ligand density will be presented.