| AVS 56th International Symposium & Exhibition | |
| Biomaterial Interfaces | Monday Sessions |
| Session BI-MoA |
| Session: | Protein and Cell Interactions at Interfaces I |
| Presenter: | X. Cui, The Scripps Research Institute |
| Authors: | X. Cui, The Scripps Research Institute HB. de Laat, The Scripps Research Institute J. Orje, The Scripps Research Institute Z.M. Ruggeri, The Scripps Research Institute |
| Correspondent: | Click to Email |
The mechanism of platelet-adhesion plays an important role in hemostasis and thrombosis. When blood vessels are injured or disrupted, the platelets membrane presented glycoprotein Ibα (GPIbα) will bind to von Willebrand Factor (vWF), which is a large multimeric plasma protein immobilized on collagen fibers. The binding interactions are regulated by the applied fluid shear rates. In this paper, we conducted a study of blood platelets adhesion on vWF A1 domain coated glass slides at different shear rates. The platelets movements are recorded by a high speed camera at 30fps. The recorded videos are analyzed using video/image processing software in order to calculate the platelet velocities at different shear rates as well as different protein coating concentrations. We found the average velocity decreased when the shear rate increased. This showed the binding requires high fluid shear rate in the flow. PLGA beads with the shapes of ellipsoid and sphere of about 1µm diameter were also studied using this method. However, the beads were coated with vWF A1 domain and the flow chambers were coated with GPIbα. We observed the decrease of the velocity with the increased shear rates, which showed increased binding strength between vWF A1 domain and GPIbα protein at higher shear rates. The ellipsoid beads had lower velocity comparing with the sphere beads at the same shear rate due to the larger contact area to the coating surface.