| AVS 56th International Symposium & Exhibition | |
| Biomaterial Interfaces | Wednesday Sessions |
| Session BI+AS+NS-WeA |
| Session: | Quantitative Nanoscale Sensing at Biosurfaces and Interfaces |
| Presenter: | M. Jonsson, Chalmers University of Technology, Sweden |
| Authors: | M. Jonsson, Chalmers University of Technology, Sweden A. Dahlin, Chalmers University of Technology, Sweden P. Jönsson, Chalmers University of Technology, Sweden S. Petronis, Chalmers University of Technology, Sweden F. Höök, Chalmers University of Technology, Sweden |
| Correspondent: | Click to Email |
The resonance condition for excitation of plasmons associated with metal nanostructures is highly sensitive to changes in the interfacial refractive index, which has made the phenomenon highly popular as transducer principle for label-free sensing of biomolecular recognition reactions. There is a particular need for sensor concepts that are compatible with studies of the cell membrane, which can be explained from the fact that more than half of the most commonly used drugs are directed towards membrane-associated reactions. This is also relevant with respect to diagnostics of viral diseases, because viruses typically infect host cells via adsorption to the cell membrane. During the past years we have developed nanoplasmonic biosensing platforms that are compatible with studies of artificial cell membranes, such as lipid vesicles and supported lipid bilayers (SLBs).[1-3] In addition to probing specific binding of ligands to membrane receptors, we showed that nanoplasmonic sensors provide a unique means to probe biomolecular structural changes, such as during the formation of a SLB from adsorption and rupture of lipid vesicles.[1]
We have previously used a metal film perforated with nanoholes as an electrode for combined nanoplasmonic and quartz crystal microbalance measurements.[3,4] Besides two independent measures on biomolecular structural changes, the combined sensor setup was shown to provide new information that enabled the quantification of adsorbed mass on the sensor surface with only the density of the molecules as unknown parameter.[3]
In the current work we utilize the continuity of a perforated plasmon active metal film to fabricate nanoplasmonic pores with liquid access to both sides of the nanopores.[5] This structure opens up for a wide range of novel applications. For example, extending our previous work on plasmonics and cell membrane mimics, an appealing possibility is to measure transport of both charged and non-charged molecules through lipid membranes that span the pores. Plasmonic pores can also be used for flow-through sensing, where flowing the target molecules through the pores will facilitate molecules to reach the sensor surface in an efficient way and circumvent limitations due to mass-transport.[6]
References:
1 Jonsson, M. P. et al. Nano Letters 2007, 7, 3462-3468.
2 Dahlin, A. B.; Jonsson, M. P.; Höök, F. Advanced Materials 2008, 20, 1436-1442.
3 Jonsson, M. P.; Jönsson, P.; Höök, F. Analytical Chemistry 2008, 80, 7988-7995.
4 Dahlin, A. B.; et al. ACS Nano 2008, 2, 2174-2182.
5 Jonsson M. P. et al. Manuscript in preparation
6 Eftekhari F. et al. Analytical Chemistry 2009, ASAP