AVS 53rd International Symposium
    Nanometer-scale Science and Technology Thursday Sessions
       Session NS-ThP

Paper NS-ThP7
Nano-Pocket Array for Protein Immobilization

Thursday, November 16, 2006, 5:30 pm, Room 3rd Floor Lobby

Session: Nanoscale Science and Technology Poster Session
Presenter: K.J. Kim, Korea Research Institute of Standards and Science
Authors: K.J. Kim, Korea Research Institute of Standards and Science
W.-J. Kim, Korea Research Institute of Standards and Science
D.H. Ha, Korea Research Institute of Standards and Science
S.I. Park, Korea Research Institute of Standards and Science
W.S. Yun, Korea Research Institute of Standards and Science
H.J. Kang, Korea Research Institute of Bioscience and Biotechnology
S.J. Chung, Korea Research Institute of Bioscience and Biotechnology
Correspondent: Click to Email
We report on the preparation of nanoscale pockets on a solid surface for protein immobilization. The nano-pockets were fabricated by e-beam lithography followed by reactive ion etching. Diameter and depth of the nano-pockets were both in the range of 30-150 nm. For some samples, thin gold layer was formed at the bottom of the pocket. The nano-pocket was first treated by either aminoalkylsilane or aminoalkylthiol depending on the material in the pocket. The pocket was then treated by succinic anhydride, NTA (Nitriloacetic acid), and Ni2+. His-tagged proteins such as GFP (green fluorescence protein) and PTP (protein tyrosine phosphatase) were then immobilized in the pocket by Ni-NTA. The fluorescence intensity of GFP and the catalytic activity of PTP were measured at intervals of a day to examine the effect of the nano-pocket on the protein stability.