AVS 53rd International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI2-TuM

Invited Paper BI2-TuM4
Single Nanopores for the Detection and Characterization of DNA and Anthrax Toxins

Tuesday, November 14, 2006, 9:00 am, Room 2014

Session: Biodiagnostic Innovation
Presenter: J.J. Kasianowicz, NIST
Authors: J.J. Kasianowicz, NIST
B. Nablo, NIST
M. Misakian, NIST
S.E. Henrickson, NIST
K. Rubinson, NIST
T. Nguyen, NCI
R. Gussio, NCI
K.M. Halverson, USAMRIID
S. Bavari, USAMRIID
R.G. Panchal, USAMRIID
V.M. Stanford, NIST
Correspondent: Click to Email
We are using electrophysiology, molecular biology and computer modeling to better understand how biopolymers (e.g., single-stranded DNA and proteins) partition into and transport through nanometer-scale pores that are formed by bacterial toxins. The results provide insight into the mechanism by which these toxins work in-vivo. They are also the physical basis for several potential nanobiotechnology applications including rapid DNA sequencing, sensitive and selective detection of a wide range of target species, high throughput screening of therapeutic agents against several anthrax toxins, and detection of anthrax lethal toxins in blood. Supported in part by NIST, the NIST Advanced Technology Program, the NIST Office of Law Enforcement Standards, and NSF.