AVS 53rd International Symposium
    Biomaterial Interfaces Wednesday Sessions
       Session BI-WeM

Paper BI-WeM13
Bioarrays with 10,000 Functionalized Spots per Square cm

Wednesday, November 15, 2006, 12:00 pm, Room 2014

Session: Bio-interfacial Modification and Bio-Immobilization I (Honoring Marcus Textor, ETH-Zürich for Substantial Contributions to the Field)
Presenter: M.R. Linford, Brigham Young University
Authors: M.R. Linford, Brigham Young University
M.C. Aplund, Brigham Young University
F. Zhang, Brigham Young University
G. Saini, Brigham Young University
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We describe here a method for very rapidly patterning surfaces to make bioarrays. In this method, a silicon surface is first coated with a monolayer to make it hydrophobic. A microlens array is then positioned over the surface and a single shot (4 ns) from a YAG laser is directed through it. The microlens array focuses the laser pulse into a myriad of tiny spots on the surface that cause removal of the monolayer, leaving ca. 20 micrometer hydrophilic spots. The density of these hydrophilic spots is 10,000 per square cm, i.e., the center-to-center distance between the spots is 100 micrometers. It is then shown by fluorescence and time-of-flight secondary ion mass spectrometry (ToF-SIMS) that polylysine will preferentially adsorb onto the hydrophilic spots. Bioconjugate chemistry from this polylysine base, e.g., the use of phenylenediisothiocyanate as a crosslinker, then allows amine-terminated oligonucleotides and proteins to be attached at the functionalized spots. This bioconjugate chemistry is confirmed on larger, planar surfaces by wetting, optical ellipsometry, X-ray photoelectron spectroscopy, ToF-SIMS, and fluorescence.