AVS 53rd International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP9
Proteins Patterning on Plasma PEG Surfaces by Microcontact Printing

Tuesday, November 14, 2006, 6:00 pm, Room 3rd Floor Lobby

Session: Biomaterial Interfaces Poster Session
Presenter: A. Ruiz, European Commission, Institute for Health and Consumer Protection, Italy
Authors: A. Ruiz, European Commission, Institute for Health and Consumer Protection, Italy
L. Ceriotti, European Commission, Institute for Health and Consumer Protection, Italy
F. Brétagnol, European Commission, Institute for Health and Consumer Protection, Italy
D. Gilliland, European Commission, Institute for Health and Consumer Protection, Italy
H. Rauscher, European Commission, Institute for Health and Consumer Protection, Italy
P. Colpo, European Commission, Institute for Health and Consumer Protection, Italy
F. Rossi, European Commission, Institute for Health and Consumer Protection, Italy
Correspondent: Click to Email
Arrays of surface-bound biomolecules are significantly needed in a variety of applications, such as diagnostic immunoassays, DNA microarrays, cell culturing or biosensing. Among all known techniques, soft lithography is a printing processes used for the fabrication of micro and nano structures based on a physical contact between a stamp and a substrate. The most representative technique of this technology is the microcontact printing. This technique is based on the use of PDMS stamps with micropatterned relief inked by biomolecules that are transferred to a substrate. Surfaces with biomolecules localised in a well arrangement can be used for cell growth studies and for improving the understanding of their interactions with different cell types. In this framework, we have used microcontact printing for patterning PEG surfaces with Poly-L-Lysine and BSA. We used the fact that PEG is known to be antifouling, i.e. protein repellent, in solution but accept protein adhesion when printed in dried conditions, as previously suggested by Delamarche.@footnote 1@ The proteins stamping is done in dried conditions and the stability of the protein patterns when put in solution has been proven. Characterisation by Fluorescence Microscopy, ToF-SIMS and Ellipsometry showed well-defined stable motifs even after 24h in water. The PLL microstamped PEG surfaces were then incubated with a solution of L929 fibroblasts. It was clearly observed how the cells adhered and grew in the regions patterned with PLL. Controlled patterning of biomolecules on an antifouling substrate like PEG has been achieved by microcontact printing technique, which also offers the advantage of being a simple, convenient, inexpensive and accessible method. @FootnoteText@ @footnote 1@ E. Delamarche, C. Donzel, F.S. Kamounah, H. Wolf, M. Geissler, R. Stutz, P. Schmidt-Winkel, B. Michel, H.J. Mathieu, K. Schaumburg. Langmuir 19 (2003) 8749 - 8758.