AVS 53rd International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP6
Interface Biology at Structured Surfaces of Titanium Dental Implants

Tuesday, November 14, 2006, 6:00 pm, Room 3rd Floor Lobby

Session: Biomaterial Interfaces Poster Session
Presenter: H.P. Wiesmann, University M@um u@nster, Germany
Authors: H.P. Wiesmann, University M@um u@nster, Germany
L. Lammers, University M@um u@nster, Germany
F. Abusua, University M@um u@nster, Germany
U. Joos, University M@um u@nster, Germany
Correspondent: Click to Email
In the present investigation three comparable threaded dental titanium implants were evaluated for the bone cell reactions at the surface in cell culture and after insertion in the mandible of minipigs. Implants with comparable size and geometry but various surface structures were used; TBS surfaces, sandblasted - acid-etched (SLA) surfaces, and microgrooved surfaces. Osteoblast-like cells were seeded under defined culture conditions on the implants and cell reactions were investigated during a 14-day culture period. For the in vivo experiments implants were placed in the mandible of 10 minipigs. Light- and electron microscopy as well as energy dispersive x-ray analysis were used. Cultured osteoblasts attached to all tested surfaces. TBS and microgrooved surfaces showed significantly more attached cells after 1 day. Proliferation of cells was best on microgrooved surfaces followed by TBS- and SLA-surfaces. After 14 days a high expression of osteocalcin, osteonectin, fibronectin, collagen type I, and osteopontin of osteoblasts cultured on microgrooved surfaces was demonstrated, whereas a lower level was present on SLA surfaces. In vivo, the most prominent difference at the interface between the implant systems was the extent of titanium wear. Number and size of titanium particles in the vicinity of the implantation bed were high around TBS-implants and low around microgrooved implants. SLA-implants showed many but small titanium particles in the interface region. In conclusion, grooved surfaces offer under in vitro conditions a better cell attachment and proliferation as well as a higher expression of typical bone related matrix proteins than the other surfaces studied.