AVS 53rd International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP22
Nano-Sphere Lithography Based Chemical Nano-Patterns for Biosensor Design

Tuesday, November 14, 2006, 6:00 pm, Room 3rd Floor Lobby

Session: Biomaterial Interfaces Poster Session
Presenter: A. Valsesia, European Commission-JRC-IHCP, Italy
Authors: A. Valsesia, European Commission-JRC-IHCP, Italy
P. Colpo, European Commission-JRC-IHCP, Italy
P. Lisboa, European Commission-JRC-IHCP, Italy
F. Rossi, European Commission-JRC-IHCP, Italy
Correspondent: Click to Email
Patterning bio-molecules on biosensor platform surfaces is a cornerstone fabrication step for many applications ranging from medical diagnostics, environmental monitoring, food safety, and security applications to more fundamental themes such as cell-surface interactions. Many works are performed worldwide to develop advanced platforms with controlled surface chemistry and well defined nano-patterns. The goal is to be able to immobilize the biomolecules in an active state, avoiding non specific adsorption, in this manner the sensitivity and the specificity of detection is enhanced by surface densification of the recognition agents. An important consequence is the reduction of analyte volume needed for the detection. Among the different nanopatterning techniques nano-sphere lithography is a very flexible technique to produce nano-structured and chemically nano-patterned surfaces. Moreover this technique presents the advantage to be inexpensive and enable to produce nano-topography over large area surfaces. In this work, we present the fabrication strategy and the surface characterization of different types of nano-structures. In particular Poly Acrylic acid (carboxylic functional) nano-domes in anti-fouling matrix have been fabricated by combining colloidal lithography and Plasma Enhanced Chemical Vapor Deposition and carboxylic terminated nano-spots in an anti-fouling matrix have been produced by combining nano-sphere lithography and Self Assembled Molecular Monolayers on gold. We show that these chemical nano-patterns are able to immobilize proteins selectively in the carboxylic functional nano-domains, leaving the anti-fouling matrix clear. Moreover immunoassay experiments were set-up showing that nano-patterned surface constrains the immobilization of the antibodies in a biological reactive configuration, thus significantly improving the device performances as compared to more conventional non-patterned surfaces.