AVS 53rd International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP21
Control of Cell Shape, Cytoskeleton Structure, and Cell Migration Activity by using a Micro 3D Patterned Film

Tuesday, November 14, 2006, 6:00 pm, Room 3rd Floor Lobby

Session: Biomaterial Interfaces Poster Session
Presenter: H. Sunami, Hokkaido University, Japan
Authors: H. Sunami, Hokkaido University, Japan
E. Ito, CREST, Japan Science and Technology Corporation (JST)
M. Tanaka, Hokkaido University, Japan
S. Yamamoto, Hokkaido University, Japan
M. Shimomura, Hokkaido University, Japan
Correspondent: Click to Email
Recently we found that endothelial cells can proliferate rapidly on a micro 3D patterned film (honeycomb film). The cell shape and cytoskeleton structure on the honeycomb films were clearly different from those on a flat film. In order to elucidate the effect of honeycomb films as a 3D scaffold for cell culture, it is needed that the 3D observation of cell behaviors such as the morphological change, expression of cytoskeleton, expression of contact points on extracellular adhesion molecules, and migration on the honeycomb films during cell culture. In this research, effects of 3D honeycomb pattern on above cell behaviors were observed. The honeycomb films were fabricated by applying a moist air to a spread polymer solution containing an biodegradable polymer (poly(@epsilon@-caprolactone)(PCL)) and an amphiphilic polymer. The regular honeycomb structures with pore diameter of 10 µm and a wall thickness of 10 - 12 µm were prepared. The porcine vascular endothelial cells were cultured on the honeycomb films for 24 h at 37 °C. The cells were slender on the flat film, while the cells were spread widely on the honeycomb film. The cell migration activity on the honeycomb films was much lower than that on the flat film. The results of confocal laser scanning microscopic observation of CFSE-stained cytoplasm, the 3D shape of the cells on the honeycomb film was different from that on the flat film. Although the cells top-surfaces on both the honeycomb films and the flat films were flat equally, the bottom surface of cell body on the honeycomb films flagged approximately 1 - 3 µm along the honeycomb pores. The focal contact points (vinculin clusters) and actin cytoskeletons expressed strongly along the pores, too. These results showed the 3D structure of the honeycomb film effects on the cell shape, migration, and cytoskeletons. We will also report the relation between the 3D structure of honeycomb film and the good cell proliferation on the honeycomb film.