AVS 53rd International Symposium
    Biomaterial Interfaces Thursday Sessions
       Session BI+AS-ThA

Paper BI+AS-ThA4
Study of the Interfacial Water Structure on Sulfobetaine-Terminated Thiolate Self-Assembled Monolayers

Thursday, November 16, 2006, 3:00 pm, Room 2014

Session: Biomolecule-Surface Characterization I
Presenter: M.J. Stein, University of Washington
Authors: M.J. Stein, University of Washington
B.D. Ratner, University of Washington
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The foreign body response to prosthetic devices limits the extended use of virtually all medical implants and biosensors. Non-specific protein adsorption is believed to be a key determinant of this response. To circumvent or control these reactions, our initial study utilized a zwitterionic sulfobetaine thiol, structurally similar to taurine (HS(CH@sub 2@)@sub 11@N(CH@sub 3@)@sub 2@@super +@CH@sub 2@CH@sub 2@CH@sub 2@SO@sub 3@@super -@), and diluted it with hydrophobic and hydrophilic thiols to determine whether the nonfouling ability of the sulfobetaine self-assembled monolayers (SAMs) could be enhanced by either an improved packing of its bulky headgroup or through an increase in the internal hydrophilicity of the thiol monolayer. In our current study, we hypothesized that the diluted groups that were previously shown to be the most nonfouling would exhibit more structured water (~3200 cm@super -1@) versus free water (~3400 cm@super -1@)) . For this study, attenuated total reflectance (ATR) was utilized to characterize changes in the water peak signal intensity through a full time-series of dilutions at multiple temperatures. Initial results have shown that a trend is present that mirrors the earlier protein adsorption results and that this trend follows a time-dependant pattern.