AVS 53rd International Symposium
    Biomaterial Interfaces Thursday Sessions
       Session BI+AS-ThA

Paper BI+AS-ThA1
Nanostructured Titanium Surfaces for Bone Biotemplating Applications

Thursday, November 16, 2006, 2:00 pm, Room 2014

Session: Biomolecule-Surface Characterization I
Presenter: K.C. Popat, University of California, San Francisco
Authors: K.C. Popat, University of California, San Francisco
T. Desai, University of California, San Francisco
Correspondent: Click to Email
A major goal in orthopaedic biomaterials research is to design implant surfaces which will enhance osseointegration in vivo. Several micro as well as nanoscale architectures have been shown to significantly affect the functionality of bone cell i.e. osteoblasts. In this work nanotubular titania surfaces fabricated by a simple anodization process were used as templates for culturing osteoblasts. The size of nanotubes can be controlled by varying the voltage and the time of anodization. Marrow stromal cells were isolated from rat and were seeded on nanotubular titania surfaces along with control surfaces. Cell adhesion, proliferation and viability were investigated for up to 7 days of initial culture culture. The cells were then provided with differentiation media to induce matrix production. The alkaline phosphatase activity and matrix production were quantified using a colorimetric assay and X-ray photoelectron spectroscopy (XPS) for up to 4 weeks of culture (3 weeks after providing differentiation media). Further, scanning electron microscopy (SEM) was used to investigate osteoblast morphology on these nanotubular surfaces.