AVS 53rd International Symposium
    Applied Surface Science Wednesday Sessions
       Session AS+BI-WeA

Paper AS+BI-WeA4
Complementary Application of SIMS and CARS for Biochemical Imaging of Cells and Tissues

Wednesday, November 15, 2006, 3:00 pm, Room 2005

Session: Imaging and Characterization of Biological Materials
Presenter: D.W. Moon, Korea Research Institute of Standards and Science
Authors: D.W. Moon, Korea Research Institute of Standards and Science
T.G. Lee, Korea Research Institute of Standards and Science
E.S. Lee, Korea Research Institute of Standards and Science
J.Y. Lee, Korea Research Institute of Standards and Science
J.W. Shim, AmorePacific Corporation
J.W. Kim, Seoul National University, Korea
K.W. Kim, Seoul National University, Korea
Correspondent: Click to Email
There have been significant progresses in analysis of biomolecules on surfaces using surface analysis tools such as XPS, SIMS, SPR, and FT-IR. Major demands of biosurface analysis come from DNA chips, protein chips, and surface modification for tissue engineering. However, cell physiologists and medical and pharmaceutical scientists prefer in-vitro analyses of biomolecules in live single cells and tissues in spite of technical difficulties to biochemical assays. In this presentation, we report our recent studies on 2D or 3D label-free biochemical imaging of various cells and tissues such as liver, skin, retina, and hair based on complementary use of coherent anti-Stokes Raman scattering (CARS) and SIMS. TOF-SIMS measurements are based on cluster ion bombardment such as Au3, Bi3, and C60 and CARS measurements are optimized for the C-H vibration of biomolecules in live cells and tissues. CARS showed clear C-H chemical bond specific images with 300 nm spatial resolution and 1 µm depth resolution down to 100 µm depth, revealing detailed tissue structures in the sub-cellular level without any damage problems. SIMS showed much more surface sensitive and specific biomolecular mass images with some depth profiling capabilities. The present status and the future prospect of complementary use of CARS and SIMS with sensitivity and selectivity improvement based on a broad band spectrum and cluster bombardment, respectively will be discussed for practical applications in disease diagnostics and cell and tissue based drug screening.