AVS 52nd International Symposium
    Nanometer-Scale Science and Technology Tuesday Sessions
       Session NS+BI-TuA

Paper NS+BI-TuA1
Nano-Patterned Surfaces Induce Bio-Molecules Oriented Immobilization

Tuesday, November 1, 2005, 2:00 pm, Room 210

Session: Molecular and Biological Applications of Nanostructures
Presenter: A. Valsesia, EC-JRC-IHCP Italy
Authors: A. Valsesia, EC-JRC-IHCP Italy
P. Colpo, EC-JRC-IHCP Italy
T. Meziani, EC-JRC-IHCP Italy
P. Lisboa, EC-JRC-IHCP Italy
M. Lejeune, EC-JRC-IHCP Italy
F. Rossi, EC-JRC-IHCP Italy
Correspondent: Click to Email
The immobilization of biomolecules in domains with the typical size of the nano-meter or few tenth of nano-meters is one of the most challenging issues of the actual research in the field of biosensors and biochips. In particular the ability to create nanopatterned bio-active surfaces should be addressed to improve the performances of biosensing devices and to study new fundamental problems. From the technological point of view the nano-patterned surfaces can improve or modulate the absorption of proteins, minimize their non-specific absorption, increase the active surface density. In the last few years nano-soft lithography, dip-pen lithography, nano-fountain pen lithography and colloidal lithography were able to produce nano-patterned surfaces with fouling-antifouling contrast. The selective immobilization of the biomolecules on the fouling regions was demonstrated as well as the reduction of the nonspecific absorption in the antifouling matrix. In this work we developed a nano-patterning method which combines the spontaneous formation of molecular monolayers (SAM) and plasma based colloidal lithography. By this approach we have shown that the nano-patterning resolution is not limited in principle and can be accurately controlled by the plasma processing parameters. The techniques was employed for the creation of chemical nano-patterned surfaces with 100 nm motives with a hexagonal 2-D crystalline structure, characterized by COOH terminated SAM nano-spots in a CH3 terminated thiols matrix. By combining the information arising from the different characterization techniques, it was possible to demonstrate the creation of a chemical contrast with a resolution of 100 nm, without a meaningful change in the morphological properties of the surface. An effective orientation of the biomolecules immobilized on these nano-patterned surfaces was demonstrated by AFM measurements and confirmed using an ELISA antibody immobilization protocol.