AVS 52nd International Symposium
    DNA Topical Conference Monday Sessions
       Session DN-MoP

Paper DN-MoP9
Characterization of dNTP and ssDNA Attached to Diazonium-modified Surfaces

Monday, October 31, 2005, 5:00 pm, Room Exhibit Hall C&D

Session: DNA Poster Session
Presenter: D. Barbash, University of New Mexico
Authors: D. Barbash, University of New Mexico
J.E. Fulghum, University of New Mexico
G.P. Lopez, University of New Mexico
Correspondent: Click to Email
The immobilization of ssDNA to various modified substrates is utilized in wide-ranging applications including DNA microarray technology and molecular wires. Surface and near-surface characterization techniques such as x-ray photoelectron spectroscopy (XPS) and ATR-FTIR have been demonstrated to be useful in the characterization of attached nucleotides (dNTPs). In this work we use dNTP-based systems as a model for more complex systems. The dNTPs can be uniquely identified using either XPS or ATR-FTIR. Orientation of the attached dNTPs was investigated by ARXPS and found to be consistent with the expected attachment chemistry. Principal component analysis (PCA) on the ARXPS data was utilized to both enhance identification of chemical species and to confirm orientation. ARXPS, ATR-FTIR and multivariate analysis methods have been applied to the study of ssDNA attachment to diazotized surfaces. We will discuss the use of these methods, in combination with data on dNTP attachment, for identifying the orientation of attached ssDNA.