AVS 52nd International Symposium
    DNA Topical Conference Monday Sessions
       Session DN-MoP

Paper DN-MoP5
Organophosphonate Monolayers as Functionalisation for Silicon Based Biosensing Devices

Monday, October 31, 2005, 5:00 pm, Room Exhibit Hall C&D

Session: DNA Poster Session
Presenter: E. Pringsheim, Walter Schottky Institute, Technical University of Munich, Germany
Authors: E. Pringsheim, Walter Schottky Institute, Technical University of Munich, Germany
M. Dubey, Princeton University
K. Buchholz, Walter Schottky Institute, Technical University of Munich, Germany
K. Arinaga, Fujitsu Laboratories Ltd., Japan
M.D. Carolus, Princeton University
J. Schwartz, Princeton University
M. Tornow, Walter Schottky Institute, Technical University of Munich, Germany
G. Abstreiter, Walter Schottky Institute, Technical University of Munich, Germany
Correspondent: Click to Email
Planar semiconductor sensing devices based on silicon substrates have immense potential for applications such as label-free, fast, and time resolved detection of biomolecule binding events due to their great sensitivity to surface potential changes via the field effect.@footnote 1@ Phosphonate films are particularly suitable as functionalisation for such devices since they are easy-to-apply and provide for stable silicon surface derivatisation due to dense, self-assembled monolayers (SAMs) that bond strongly to the native silicon oxide. These SAMs can be modified with tailored, substituted end groups.@footnote 2@ We have investigated the covalent linkage of thiol-terminated Cy3 labelled DNA-oligonucleotides to functionalized alkylphosphonate layers assembled on silicondioxide by variation of the immobilisation parameters, including concentration of DNA, concentration of sodium chloride and immobilisation time. Hybridization with complementary strands and the coupling of the model protein avidin to biotinylated DNA is detected optically. Concepts for biofunctionalisation of field effect sensor devices via phosphonic acid monolayers will be discussed. @FootnoteText@ @footnote 1@ M. G. Nikolaides et al., ChemPhysChem, vol. 4, 1104-1106 (2003)@footnote 2@ K. S. Midwood et al., Langmuir, vol. 20, 5501-5505 (2004).