AVS 52nd International Symposium
    DNA Topical Conference Monday Sessions
       Session DN+BI-MoA

Paper DN+BI-MoA5
Electrically Switchable DNA Layers as a Novel Detection Scheme for Bio-Sensing

Monday, October 31, 2005, 3:20 pm, Room 311

Session: DNA Detection and Sensing
Presenter: U. Rant, University of Technology Munich, Germany
Authors: U. Rant, University of Technology Munich, Germany
K. Arinaga, University of Technology Munich, Germany
E. Pringsheim, University of Technology Munich, Germany
M. Grubb, University of Technology Munich, Germany
S. Fujita, Fujitsu Laboratories Ltd., Japan
N. Yokoyama, Fujitsu Laboratories Ltd., Japan
M. Tornow, University of Technology Munich, Germany
G. Abstreiter, University of Technology Munich, Germany
Correspondent: Click to Email
Recently, we reported on the electrical manipulation of oligonucleotide layers tethered to gold surfaces.@footnote 1@ By applying alternating AC potentials to the supporting substrate, the orientation of the DNA strands can be dynamically switched with frequencies ranging up to the kHz regime, while the layer conformation is probed in real-time by optical means. Here we present the underlying principles governing the switchability of DNA layers and, in particular, how this novel functionality can be employed for multi-purpose, label-free sensing applications. In principle, the method can be utilized to detect any kind of (bio-) molecules that, upon specifically binding to the grafted probe layer will alter its switching behavior. As a unique feature, the technique allows to monitor the molecular dynamics of the electrically switched layers, which provides novel means to identify and characterize target-probe complexes on surfaces. We demonstrate the versatility and high sensitivity of the technique by the recognition of specific DNA sequences as well as the detection of a model protein system. @FootnoteText@ @footnote 1@ U Rant, K Arinaga, S Fujita, N Yokoyama, G Abstreiter, M Tornow; Nano Letters 4 (2004), 2441-2445