AVS 52nd International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI2-TuA

Paper BI2-TuA1
Dynamic Ellipsometric Studies of Protein Adsorption to Modified Chitosan Surfaces

Tuesday, November 1, 2005, 2:00 pm, Room 312

Session: Surface Modification
Presenter: S. Sarkar, University of Nebraska, Lincoln
Authors: S. Sarkar, University of Nebraska, Lincoln
L.G. Castro, University of Nebraska, Lincoln
D.W. Thompson, University of Nebraska, Lincoln
J.A. Woollam, University of Nebraska, Lincoln
A. Subramanian, University of Nebraska, Lincoln
Correspondent: Click to Email
Protein adsorption is a ubiquitous phenomenon whose effects are widespread and observable in fields as diverse as biofouling, molecular recognition and metabolic pathway activation (biocompatibility) and even qualitative and quantitative detection methods (ELISA). Protein interaction with materials and at interfaces is an area of ongoing study; however, little is understood of the immediate response of proteins to an exposed surface. Chemically modified chitosan films were used to investigate the adsorption of human serum albumin (HSA), immunoglobulin G, and fibrinogen. Diepoxides were reacted with amine groups present on chitosan and then five atomic geometries (n-butyl amine, 2-(t-Butylamino)ethanol, n-octyl amine, 2,4,6 Tris(dimthylaminomethyl) and t-butyl amine) and an anti-HSA molecule were attached to free epoxide ends to create surfaces preferential to albumin adsorption. Dynamic ellipsometric studies were carried out on the resulting surfaces to investigate protein adsorption phenomena. We have been successful in observing real time protein adsorption. In most cases protein adsorption had reached a saturation point after one-hour with the highest rate of adsorption occurring in the first ten minutes.