AVS 52nd International Symposium
    Biomaterial Interfaces Thursday Sessions
       Session BI-ThA

Paper BI-ThA5
Fabrication of Bioconjugated Polymeric Nanostructures and Metal Nanowires by AFM Anodization Lithography

Thursday, November 3, 2005, 3:20 pm, Room 311

Session: Bionanotechnology
Presenter: S. Zauscher, Duke University
Authors: W.-K. Lee, Duke University
H. Ma, Duke University
S. Chen, Duke University
A. Chilkoti, Duke University
S. Zauscher, Duke University
Correspondent: Click to Email
Patterning of polymeric and biomolecular nanostructures on surfaces and the control of their architecture are critically important for the fabrication of biomolecular devices and sensors. Here we show for the first time how we use AFM anodization lithography to chemically modify polymer brushes directly to allow conjugation of biomolecules. Surface-confined non-fouling and protein resistant poly(oligo(ethylene glycol) methyl methacrylate) (pOEGMA) brushes were prepared on silicon substrates by surface-initiated atom transfer radical polymerization (ATRP) in a grafting-from approach. These pOEGMA brushes were then patterned directly on the nanoscale by AFM anodization lithography, generating nano-trenches with carboxylic acid functionality. Proteins were then immobilized on these nanopatterned areas by suitable coupling chemistries. We also show an intriguing approach to deposit gold onto silicon oxide patterns by field-emission from gold-coated AFM probes. We capitalize on this novel lithography approach to fabricate gold nanowires of arbitrary shape. Our unique nanofabrication approaches lead to novel types of nanostructures that can potentially be used as biosensors or as substrates for the precise presentation of biomolecular queues to cells. Furthermore, our gold nanostructures can be used for electrical connections, or as plasmonic structures for biomolecular sensing.