AVS 52nd International Symposium
    Biomaterial Interfaces Thursday Sessions
       Session BI-ThA

Paper BI-ThA4
Dynamic Surface Modification and Patterning using Electrochemistry and Molecular Assembly Approach

Thursday, November 3, 2005, 3:00 pm, Room 311

Session: Bionanotechnology
Presenter: C.S. Tang, Swiss Federal Laboratories for Materials Testing and Research (EMPA), Switzerland
Authors: C.S. Tang, Swiss Federal Laboratories for Materials Testing and Research (EMPA), Switzerland
S. Makohliso, Ayanda Biosystems SA
M. Heuschkel, Ayanda Biosystems SA
J. Voeroes, Swiss Federal Institute of Technology (ETH)
S. Sharma, Swiss Federal Institute of Technology (ETH)
B. Keller, Swiss Federal Laboratories for Materials Testing and Research (EMPA), Switzerland
M. Textor, Swiss Federal Institute of Technology (ETH)
Correspondent: Click to Email
Microarray technology is a powerful and versatile tool commonly used in biochemistry and molecular biology. This miniaturized and parallelized technique has contributed significantly to bioanalytical processes such as large-scale genomic sequencing. One option for additional flexibility within a microarray is the use of electrochemical tools to dynamically influence and steer formation and properties of adsorbed molecular layers at the solid-liquid interface. By controlling and manipulating the placement of polyelectrolytes and biomolecules under the influence of an electric field, we have demonstrated that an electroactive biosensing platform with specificity and high sensitivity enable rapid screening and discrimination of different biomolecules with high selectivity. Using patterned substrates consisting of conductive areas in a non-conductive background, the electrically switchable surface can be modified to reversibly adsorb and release an adlayer of protein-resistant polymer. Macromolecules or biomolecules could be subsequently adsorbed onto the polarized indium tin oxide (ITO) microelectrodes by using simple surface chemistry. As a proof of concept, labeled functionalized polymer, proteins and vesicles were immobilized onto the ITO microelectrodes to produce a highly selective and heterogeneous microarray with specific electronic addressability. Some future applications with a localized addressable electronic microarray could include microfluidics, biosensors, drug delivery and manipulation of cellular neuron network for tissue engineering.