AVS 52nd International Symposium
    Biomaterial Interfaces Monday Sessions
       Session BI-MoP

Paper BI-MoP38
Sensitivity Enhanced Biosensor by Prussian Blue Modified Electrode

Monday, October 31, 2005, 5:00 pm, Room Exhibit Hall C&D

Session: Biomaterial Interfaces Poster Session
Presenter: I.J. Yi, Myongji University, Korea
Authors: I.J. Yi, Myongji University, Korea
J.H. Kim, Myongji University, Korea
C.J. Kang, Myongji University, Korea
Y.S. Kim, Myongji University, Korea
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We propose a sensitivity enhanced biosensor by Prussian blue (PB) modified indium tin oxide (ITO) electrode. A PB film plays a significant role of electron-transfer. Capillary electrophoresis (CE) and amperometric method were adapted to our work. Microchip was fabricated with polydimethylsiloxane (PDMS) to form microchannels and ITO patterned glass in inexpensive and simple method. The PB film was electrodeposited on the working electrode of various conditions to obtain stable PB film. Atomic force microscopy (AFM) was used to observe the changes of PB film surface. Calibrated PB film deposition voltage and time were obtained by AFM topography which shows PB surface characteristics. The optimized thickness of stable PB film was obtained when deposition voltage was about 0.1 V for 3 min. We detected various concentrations of neurotransmitters (dopamine and catechol) and hydrogen peroxide which is for detecting glucose respectively. It is observed that there was 20 times higher peak current for PB/ITO electrode than that for previously reported ECDs using bare-ITO electrode.@footnote 1@ Results are indicating rapid separation and detection of the analytes. The measured peaks of dopamine, catechol and hydrogen peroxide were proportional to their concentrations. When PB-modified electrode was used, the sensitivity was improved compared with bare-ITO electrode. It is believed that the PB film can be a viable candidate for a disposable and sensitive biosensor. @FootnoteText@ @footnote 1@Ju-Ho Kim, C. J. Kang, and Yong-Sang Kim, "A Development of a Microfabricated Disposable Microchip with a Capillary Electrophoresis and Integrated Three-Electrode Electrochemical Detection", Biosensors & Bioelectronics, vol.20, no.11, p.2314-2317, (2005).